Phospho s536
WebNational Center for Biotechnology Information WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) Research with …
Phospho s536
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WebOct 1, 2004 · Phosphorylation of p65 at S536 is required for NF-κB/p65 transactivation and also influences p65 DNA binding and recruitment of p300. Lack of sufficient p65 phosphorylation at S536 in P− cells results from deficient ERK content, which in turn contributes to a low level of cellular IKKβ. http://www.immunoway.com/Home/22/YP0191
WebNo lysate preparation required. Results with as few as 10,000 cells per well. Measure two proteins simultaneously in the same well. Ideal for measuring phosphorylation. Design allows for normalization of well-to-well variations. Complete kits require minimal optimization. Suitable for high-throughput analysis of non-adherent cells. WebOct 2, 2024 · (BS4138) NFkB-p65 (phospho-S536) polyclonal antibodyQ04206 - Bioworld - CiteAb. 14 citations – A rabbit polyclonal antibody, raised against Transcription factor p65 (Homo sapiens), supplied by Bioworld. ... Phospho (Ser536) View Product On Supplier's Website. Add to Procurement List Product is on your procurement list. View List. Remove.
WebOct 14, 2005 · In addition, the phospho-p65 (Ser(536)) was not associated with either IkappaBalpha or p50, and the nuclear translocation of phospho-p65 (Ser(536)), but not total p65, was unaffected by the proteosome inhibitor MG-132, which blocks IkappaB protein degradation and prevents p65/p50 dimer nuclear translocation. WebAug 12, 2024 · The protein expression of pIκB, IL-1β, IL-6, NFκB p65 (phospho S536) and TNF-α in colonic tissues was examined using Western blot analysis. Representative data are demonstrated (C) and the relative protein intensities of pIκB, IL-6, IL-1β, NFκB p65 (phospho S536) and TNF-α were normalized to GAPDH (D–H) .
WebJan 31, 2024 · Whole cell lysates were immunoblotted for phospho-S536 p65, phospho-S276 p65, total p65, phospho-S176/180 IKKα/β, total IKKα/β, phospho-IκBα, total IκBα, and β-actin. (B, C) IL-2-primed NK cells were mixed with autologous target cells and stimulated with plate-immobilized aKIR and/or aPD-L1 for 1 h. Cytoplasmic and nuclear extracts ...
WebThe antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser536. AA range:502-551. Specificity：. Phospho-NFκB-p65 (S536) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when phosphorylated at S536. Formulation：. inboxdollars an error has occurredWebAntibody was affinity purified using an epitope specific to Phospho RelA (S536) immobilized on solid support. The epitope recognized by A302-062A maps to a region of human v-rel … inclination\\u0027s kmWebEu-phospho-GSK3b (5 µL) Acc-phospho-GSK3b (20 µL) GSK3b control lysate (100 µL) 5X Lysis Buffer 3 (1 mL) 10X TR-FRET Detection buffer (50 µL) 100X Phosphatase Inhibitor Cocktail (50 µL) Reactivity Human US $ 382.00 Add to cart BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of … inclination\\u0027s kkWebThe anti-IgG and the two phospho-specific antibodies recognizing p65 phosphorylated at serine 468 (3039S) or serine 536 (3031S) were from Cell Signaling Technology. Secondary horseradish peroxidase coupled anti-rabbit or anti-mouse antibodies were … inboxdollars affiliateWebTHUNDER™ Phosphatase Inhibitor Cocktail (100X) THUNDER™ Phospho-NF-kB (S536) + Total NF-kB TR-FRET Cell Signaling Assay Kit Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-NF-κB (S536) and Total NF-κB in cell lysates. inclination\\u0027s koWebRayBio® Phospho- NF-κB p65 (S536) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated NF-κB p65 protein in your experimental model system, you can verify pathway activation in your cell ... inclination\\u0027s ksWebAntigen retrieval of a rabbit polyclonal anti-NF-κB p65 (phospho S536) was performed by incubating the sections with target retrieval solution (pH 9.0) for 20 minutes, other antibodies were performed by incubating the sections with citrate buffer (pH 6.0) for 10 minutes using pressure cooker, then the immune complex was detected with an … inboxdollars average income