Phospho eif4b
WebWestern blot analysis of extracts from HeLa cells, untransfected or transfected with SignalSilence ® eIF4B siRNA #6416, using Phospho-eIF4B (Ser422) Antibody (upper) or eIF4B Antibody #3592 (lower). 48 hours following transfection, cells were treated with Rapamycin (50 nM) and U0126 (10 uM) as indicated. WebThe eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and …
Phospho eif4b
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WebThe eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. WebPhospho-EIF4B-S422 Rabbit pAb (AP0278) Datasheet SDS Tested applications: WB IHC Reactivity: Human, Mouse Western blot analysis of extracts from HeLa cells using phospho-EIF4B-S422 antibody (AP0278). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
WebAnti-eIF4B (phospho S422) antibody See all eIF4B primary antibodies Description Rabbit polyclonal to eIF4B (phospho S422) Host species Rabbit Tested applications Suitable for: IHC-P, WB, ICC/IF more details Species reactivity Reacts with: Mouse, Human Immunogen Synthetic peptide corresponding to Human eIF4B aa 350-450 (phospho S422). T-G-SP-E-S WebAll lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution Lane 1 : 293T cell lysate, untreated Lane 2 : 293T cell lysate, treated with AP Lysates/proteins at 10 µg per lane. Secondary All lanes : goat anti-rabbit HRP at 1/2000 dilution Predicted band size: 69 kDa Observed band size: 69 kDa
WebEIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth … WebProduct Details Target Information The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP.
WebAug 17, 2014 · Phospho-proteomics identified the mTOR pathway to be of interest in EGFR inhibitor resistance. In our studies, combining gefitinib and temsirolimus decreased cell growth and survival in a synergistic manner. Our data identified eIF4B, as a potentially key fragile point in EGFR and mTOR inhibitor synergy.
WebInvitrogen Anti-Phospho-EIF4B (Ser504) Polyclonal, Catalog # PA5-114571. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (PFA fixed) … check kidney functionWebOct 6, 2008 · Insulin stimulation resulted in phosphorylation of eIF4B as detected by both the phospho-eIF4B Ser422 antibody as well as the phospho-PKB substrate antibody (Figure 5a, lanes 1 and 2). check kforWebPhospho-eIF4B (Ser406) Antibody detects endogenous levels of eIF4B protein only when phosphorylated at Ser406. Species Reactivity: Human, Mouse. Species predicted to react … flask url_for static subdirectoryWebRecombinant Recombinant Anti-eIF4B (phospho S406) antibody [EPR7683] (ab134138) Datasheet SDS Submit a review Submit a question References (2) Key features and details Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply Rabbit monoclonal [EPR7683] to eIF4B (phospho S406) Suitable for: … flask upload to s3WebEIF4B Antibody (phospho-Ser422) LS-C826726 Antibody: EIF4B Rabbit anti-Human Polyclonal (pSer422) Antibody . Reactivity: Mouse, Rat, Human . Applications: ELISA, IF, IHC, WB . Format: Unconjugated . Price: 1 mg/$357. Select. EIF4B Antibody LS-C334046 Antibody: EIF4B Rabbit anti-Human Polyclonal Antibody ... flask used for vacuum filtrationWebSep 5, 2024 · eIF4B phosphorylation patterns in hippocampal neurons and cortical astrocytes. ( A) Western blot analysis of total cell extracts from neurons or astrocytes … flask user already existsWebJun 8, 2024 · The phosphorylation of eIF2α occurs on serine 51 in response to the activation of one out of four eIF2α kinases 4, 5, 6. EIF2AK1, commonly known as heme-regulated inhibitor (HRI), is activated by... flask used for filtration