WebThe Rf value can be easily obtained by calculating the distance between the developing solvent and the distance the compound moved. Rf value = distance moved by the compound ÷ distance moved by the developing solvent For example, in the following conditions, the Rf values are 0.6 and 0.2, respectively. Web14 jun. 2013 · Calculating Rf Values - YouTube 0:00 / 6:37 Calculating Rf Values ochemistree 1.47K subscribers Subscribe 2.4K Share 420K views 9 years ago This video is being submitted for the Khan...
Retention Factor in Chromatography Formula & Overview - Video ...
WebThe Rf is calculated as the ratio of the distance migrated by the molecule to that migrated by a marker dye-front. A simple way of determining relative molecular weight by electrophoresis is to plot a standard curve of Rf vs. logMw for known samples (ladder), and read off the logMw of the sample after measuring distance migrated on the same gel. Web7 sep. 2024 · To calculate an Rf value, divide the distance travelled by the component – in other words, the distance from the starting pencil line to the coloured spot -by the … greatest fantasy novel series
How to Calculate the Rf Value
Web6 jun. 2024 · To calculate an Rf value, divide the distance travelled by the component – in other words, the distance from the starting pencil line to the coloured spot -by the distance travelled by the solvent. Rf values are important because each component has a fixed Rf value under a specific set of conditions. Click to see full answer Web31 aug. 2024 · The Rf value can be calculated by measuring the distance of the substance from its starting point in millimeters, as well as the distance the solvent traveled from its … WebThe Rf value, also known as the retention factor, is a measure of the position of a component in a chromatographic separation. It is calculated by dividing the distance … flipkey booking fee