Cy3 colour

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August undefined, 2024

WebApr 12, 2024 · CY3-amine作为一种常用的荧光染料，可以用于细胞成像和荧光探针方面的研究。 CY3-amine的应用非常多，可以用于检测活细胞和固定细胞内的RNA、DNA、蛋白质等分子，还可以用于细胞膜标记和荧光标记等方面。在细胞成像方面，CY3-amine可以用于荧光显微镜观察细胞的形态、结构和功能。例如，将CY3-amine标记在蛋白质上，可以观 … WebThe cyanine dye Cy3 is a popular fluorophore used to probe the binding of proteins to nucleic acids as well as their conformational transitions. Nucleic acids labeled only with Cy3 can often be ...

Alexa Fluor Dyes—Across the Spectrum - Thermo Fisher …

WebAlexa Fluor® 594. Alexa Fluor 594 conjugates (Amax 591 nm, Emax 614 nm) emit in the red range of the visible light spectrum, are brighter than other red-fluorescing dye conjugates, and allow better color separation from green fluorescent dyes like Alexa Fluor 549, Cy3, or TRITC. They are the best choice for immunofluorescence detection in the far red region … WebJul 1, 2024 · Answer. Cy3 is orange in color. It fluoresces greenish yellow at 555 nm excitation and 570 nm emission. Cy3 belongs to the Cyanine family of synthetic … emily hinson

Analysis of Fluorescent Dyes Used for Labeling DNA in Microarray ...

WebCy3 carboxylic acid, fluorescent dye, Cy3 C32H40N2O5S CID 131953006 - structure, chemical names, physical and chemical properties, classification, patents, literature, … WebAlexa Fluor® 555 vs. Cy®3 dye Alexa Fluor® 555 dye has higher photostability and quantum yield than tetramethylrhodamine isothiocyanate (TRITC) and rhodamine derivatives, which allow more time for observation and image capture. Alexa Fluor® 555 dye also has superior fluorescence output per protein or nucleic acid conjugate, surpassing … WebThe intensity falls by 55% for Cy3 and almost 70% for Cy5. The horizontal lines show the fluorescence intensity of single-stranded reference sequences on the same arrays. Fluorescence intensity consensus sequences of all 1024 dsDNA 5mers 5′-end-labeled using (B) Cy3 and (C) Cy5. The fluorescent range was equally divided into eight bins of ... emily hipps

Cyanine Dye 3 - an overview ScienceDirect Topics

Cyanine Dyes (Cy2, Cy3, and Cy5) - Jackson …

WebAlexa Fluor 555 dye dashboard. Recommended replacement for tetramethylrhodamine or Invitrogen Cy3 dye, exhibiting greater photostability than Cy3 dye in live and fixed cells. … WebCy3 Dye If the Cy3 dye is sufficiently close (2–9nm) to the Cy5 (acceptor) dye, energy transfer can occur resulting in a mixture of emitted light coming from the donor and … emily hinkler parentsWebIndocarbocyanine, Cy™3 Technical Information. Cy™3 is brighter, more photostable and gives less background than other orange-red fluorescing dye conjugates. Cy™3 conjugates can be excited maximally at 550 nm, … dragados offshore

"WebChromaTide OBEA-dCTP nucleotides have been prepared from four of our brightest and most photostable dyes—Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 594 and Alexa … " - Cy3 colour

Cy3 colour

sulfo-Cyanine3 in Fluorophores - Lumiprobe

WebNov 16, 2024 · Hello, I've recently encountered an issue with a pre-mixed PCR buffer containing cy5 as a reference dye. It seems that cy5 signal is completely absent, while … Web1 day ago · Dolomite CY3-X3 Mens Ski Boots Mondo 25.5, Sole 294mm, Downhill, Blue eBay Dolomite CY3-X3 Mens Ski Boots Mondo 25.5, Sole 294mm, Downhill, Blue Be the first to write a review. Condition: Pre-owned “*** pre-owned, in great condition! ***” Price: US $84.95 Buy It Now Add to cart Best Offer: Make offer Add to Watchlist Breathe easy.

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WebCytiva™ Description General description Cy3 ™ Maleimide Mono-Reactive Dye 5-Pack More selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions. Provides an alternative when CyDye ™ NHS Esters are unsuitable.

WebInvitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low ... WebCy3 550 570 yellow TRITC 547 572 yellow Alexa fluor 546 556 573 yellow Alexa fluor 555 556 573 yellow R-phycoerythrin (PE) 480;565 578 yellow Rhodamine Red-X 560 580 …

WebS. pombe cells were challenged with 0.5 mM hydrogen peroxide for various periods of time (0, 10, 30, 60, 120, 180 minutes). Total RNA was isolated at each time point, labeled with Cy3 and hybridized on cDNA microarrays competitively with Cy5 labeled RNA pooled from all 6 samples. A biological replicate was performed in analyzed as a dyw swap (pool = … WebApr 13, 2024 · Cyanine染料是一类含有环状共轭结构的有机染料，其分子结构中包含有两个或多个芳香族环、吡啶环、噻吩环等。这些结构的存在使得Cyanine染料在吸收和发射光谱方面表现出色，鉴于它们的分子结构中含有苯环和杂环，因此在UV-Vis吸收光谱上表现出明显的吸收峰。此外，Cyanine染料还表现出一些特殊的性质，如荧光共振能量转移和激发 …

WebThe Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the …

WebCyanine Dye 3. Usually, cyanine 3 (Cy3) and cyanine 5 (Cy5) with excitation and emission maxima wavelengths of 555/569 and 651/670 nm are respectively used to label nucleotides (usually dCTP or dUTP) and incorporated them into DNA of interest using DNA polymerase. drag acts londonWebThe color of Cy3 is pinkish red and that of Cy5 is blue to the naked eye. However emission spectrum of Cy3 is in the greenish yellow and that of Cy5 is in the Red region. emily hirsekornWebOct 9, 2024 · To examine their mitochondria targeting ability, HeLa cells were incubated with 10 μM Cy3, Cy5 or Cy3–Cy5 (Fig. 1a and b) followed by live-cell confocal imaging. Cy3–Cy5 was chosen to investigate if having two fluorophores would enhance uptake and targeting effect. This is of interest for designing Cy3–Cy5-based FRET pairs as … drag acts to hireWebMar 30, 2024 · CY3-azide的优点在于其化学性质稳定，反应条件温和，不影响生物分子的活性和功能。同时，CY3-azide的发射光谱波长在550-570nm，适合与其他荧光染料共同使用，可以实现多色成像。 Cyanine3 dye azide可以用于点击化学，是Cy3 azide的类似物。 drag actionWeb20 rows · Try this Alexa Fluor dye; AMCA, coumarin: Alexa Fluor 350: … drag actsWebCy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λ ex =554, λ em =568). For research use only. We do not sell to patients. CY3 Chemical Structure CAS No. : 146368-13-0 Get it … emily hintzmanWeb571. Quantum yield. 0.581. Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes … drag act liverpool